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SingleB® Hybridoma Protein Expression

Alpaca SingleB® VHH Discovery Service

There are naturally heavy chain antibodies (HCAb) that lack light chain and heavy chain CH1 structure but completely retain antigen binding activity in camelic animals such as alpacas. The variable region of heavy chain that specifically binds antigen is called single domain antibodies (sdAbs), or nanobody (VHH), which is the smallest unit antigen binding fragment.

Based on the flow cell sorting platform and DeepLight® screening platform, DetaiBio provides SingleB® Alpaca VHH Antibody Fast Discovery. The whole process from alpaca immunity, memory B cell and plasma cell double screening, BGE® high-throughput expression, expression identification to purification takes only 90 days, which saves at least 15 days compared with the display library.

VHH Features

Conventional antibody

Camelid heavy-chain antibody



Based on its structural specificity, VHH integrates the advantages of traditional antibodies and small molecule drugs:

  • Low Molecular Weight: only 12-14 kda with strong tissue penetration, widely used in tumor diagnosis and treatment and molecular imaging.
  • Simple Structure: easy to carry out genetic engineering transformation, with 80% homology with human VH and easy to humanize.
  • Low Immunogenicity: no FC tail end, which will not cause related immunogenicity, and has high pharmaceutical properties.
  • High Specificity: long CDR3 region , which can form a finger shaped convex ring to further identify hidden antigen epitopes.
  • High Hydrophilicity: hydrophilic amino acids in FR2 area ensure water solubility, and antibodies are not easy to aggregate.
  • Simple Preparation: it can be expressed in large quantities, and the industrial scale-up production cost is low and the cycle is short.
  • High Stability: it is easy to store and can withstand high temperature, strong acid, strong alkali and other mutagenic conditions.

Service Features

90-day High Delivery

Deliver 50 FACs binders
& VHH sequence

“1+1”Double Guarantee

double screening, double guarantee

Pre-function Screening

DeepLight® Platform
with multiple on-chip assay

Natural Maturation

Higher antibody affinity
Basically eliminate immunogenicity



Stage Ⅰ Animal Immunization
  • Antigen preparation (optional)
  • Animal immunization
  • Valence determination
8-9 weeksPremium Package

  • FACs binders >50
  • Ensure sequence diversity
  • Antibody sequence and expression plasmid
  • CoA

Economic Package

  • 5-10 ELISA positive clones
  • Antibody sequence and expression plasmid
  • Select one strain purified 1 mg antibody
  • CoA
Stage Ⅱ “1+1”Double Screening
  • MBC screening
  • PBC screening
1 day
Stage Ⅲ BGE® HTP Expression & Functional Test
  • ELISA Assay
  • FACs Assay
1-2 weeks
Stage Ⅳ Monoclonal Antibody Gene Sequencing
  • HTP gene screening
1 week
Stage Ⅴ
Expression and Purification of Recombinant Antibody (optional)
  • Transfection level plasmid preparation
  • Transient transfection expression
  • HTP purification
  • Endotoxin control
For enquiry

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