Phosphorylation Specific Antibody Discovery Service
Phosphorylation specific antibodies are critical research tools that enable scientists to detect, differentiate, and quantify phosphorylated versus non-phosphorylated forms of proteins. These antibodies target phosphorylated amino acid residues—such as phospho-serine, phospho-threonine, and phospho-tyrosine—providing unparalleled specificity in studying cell signaling pathways, protein regulation, and disease mechanisms.
At DETAIBIO, we combine advanced SingleB® Rapid Monoclonal Antibody Platform technology with an expert CRO service model to deliver high-quality, validated phosphorylation specific antibodies. We provide both mouse monoclonal antibody production and rabbit monoclonal antibody production with guaranteed specificity confirmed by cross-validation using both phosphorylated and non-phosphorylated peptides.
Why Choose DETAIBIO for Phosphorylation Specific Antibody Discovery?
- Specialized Expertise – Over a decade of experience in phosphorylation specific immunogen design and synthesis.
- High-Throughput Screening – Proprietary B cell screening technology for rapid discovery of high-affinity clones.
- Guaranteed Specificity – Final antibodies validated against both phosphorylated and non-phosphorylated peptides.
- CRO-Focused Service – Fully customizable project plans to fit pharmaceutical, biotech, and academic research needs.
Service Workflow
| Service Stage | Service Details | Deliverables |
|---|
| Immunogen Design & Synthesis (Optional) | Synthesis of phosphorylated and non-phosphorylated peptides | - 3 ELISA-positive clones
- Project report
|
| Peptide Conjugation (Optional) | Conjugation of peptides to carrier proteins (KLH, BSA) |
| Animal Immunization | - Animal immunization
- Titer measurement
|
| B Cell Screening | Memory B cell screening |
| BGE® High-Throughput Expression & Functional Validation | ELISA assay |
| Monoclonal Antibody Gene Sequencing | High-throughput sequencing |
| Recombinant Antibody Expression & Purification (Optional) | - Transfection-grade plasmid preparation
- transient expression & purification
| - Select one strain purified 1 mg antibody
- Certificate of Analysis (CoA)
|
Case study
Animal Immunization & Cross detection of Serum Titer
Immunogen: Phosphorylated peptide-KLH
Screening Antigen: Phosphorylated peptide-BSA
Using cell surface markers and the target antigen, phosphorylation specific memory B cells were isolated. A total of 99 antigen-specific B cell clones were obtained.
Antibody functional verification
The obtained antibodies were subjected to ELISA assays, resulting in the final selection of six clones that met the screening criteria.
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