Product Description
Mouse Single B Cell Amplification Kit is optimized to synthesize full‑length cDNA in a single tube from 1–500 mouse B cells (or 10 pg–5 ng total RNA), and specifically amplifies the B cell receptor IgG heavy‑chain variable region and κ light‑chain variable region.
Applications
- B cell receptor (BCR) sequence analysis
- Construction of BCR variable region libraries
Product Advantages
- Suitable for 1–500 mouse B cells or 10 pg–5 ng total RNA, ideal for micro‑quantity or single‑cell samples.
- Co‑amplify both IgG heavy‑chain variable region (VH) and κ light‑chain variable region (VL).
- Broad primer design accommodates antibody variable‑region diversity across mouse repertoires.
- Heavy and light chains amplified as matched pairs in > 90 % of reactions.
- Simple three‑step workflow (RT→PCR1→PCR2), completed in under 5 hours
Kit Composition & Storage Conditions (96 Reactions)
| Component | Volume | Storage |
|---|
| dNTP Mix | 192μL | -20℃ |
| Cell Lysis Buffer | 96μL | -20℃ |
| RT Primer | 38.4μL | -20℃ |
| DTT | 96μL | -20℃ |
| Murine RNase Inhibitor | 38.4μL | -20℃ |
| Reverse Transcriptase | 38.4μL | -20℃ |
| 2 × PCR Mix | 5mL | -20℃ |
| PCR1 Primer Mix | 480μL | -20℃ |
| PCR2 mHeavy Primer Mix / PCR2 mLight Primer Mix | 96μL each | -20℃ |
| RNase-Free water | 4mL | -20℃ |
| TS Primer | 96μL | -80℃ |
| 5 × RT Buffer | 384μL | -80℃ |
User‑Supplied Materials
- Sample: mouse B cells or equivalent micro‑quantity RNA
- Equipments: shaker, mini‑centrifuge, thermal cycler, etc.
