SingleB® Hybridoma Protein Expression

Anti-idiotype Antibody Production Service

Antibody idiotypes are structural attributes of the V regions of antibody molecules that can be detected by anti-idiotype (anti-ID) antibodies. Since they are capable of binding to antibody drugs within biological fluids, anti-ID antibodies have been used ascritical reagents in pharmacokinetic (PK) and pharmacodynamics (PD) research in antibody drug development. They are also commonly used as key reagents for anti-drug antibody (ADA) studies, vaccine development, transplantation immunity and autoimmune studies, et al.

With SingleB® antibody discovery platform, as described elsewhere in this website, DetaiBio offers antibody discovery of high quality in a fast-and-easy way. Our highly sensitive and specific anti-ID antibodies can help push the boundaries of antibody drug development. This one-stop anti-ID antibody service covers procedures of antigen preparation, anti-ID antibody discovery, antibody pairing, protocol development& transfer and reagents production.

Since rabbit antibodies combine high affinity with high specificity, precise recognition and excellent stability, they hold the potential of overcoming bottlenecking problems such as lower detection limit and heavy matrix effect seen in clinical PK/PD/ADA studies with mouse antibodies, which seriously delays therapeutic antibody development.

DetaiBio provides unique rabbit monoclonal antibody discovery service based on its unparalleled expertise in single B cell screening. With our platform, rabbit antigen-specific memory B cells and plasma cells are directly screened in a single-cell level, with minimal hurt to antibody diversity to guarantee sufficient and high-quality anti-ID antibody generation.

Service Features

High Success Rate

Locking specific binding sites in more candidate antibodies

Highly Experienced

Improved kit development platform

Highly Sensitive

The sensitivity meets the needs of preclinical detection

One-stop Service

From antigen preparation to kit development and production

Types of Anti-idiotype Antibody


Monoclonal Anti-idiotype Antibody Production Service

According to the type of antibody drugs to be tested, DetaiBio can provide competitive or non competitive anti idiotypic antibody preparation services. According to the requirements of detection sensitivity, if the detection sensitivity needs to be at the nanogram level, mouse monoclonal anti idiotypic antibody can be selected. If the detection sensitivity needs to be at the peak level, rabbit monoclonal anti idiotypic antibody can be considered.

Antigen Preparation (optional)Customers can provide antibody drug sequences or antibody drug samples (purity >85%)
  • 5-10 ELISA positive clones (ensure delivery of 1 competitive or non competitive antibody)
  • Select one strain purified 1 mg antibody
  • ELISA paired antibody (optional)
  • CoA
Antigen Quality TestingSDS-PAGE analysis of antibody drug samples
Animal Immunization
  • 2-4 balb/c mice or 2 rabbits Immunization
  • Serum titer test
“1+1”Double Screening
  • Memory B cells screening
  • Plasma cells screening
BGE®HTP Expression & Functional Test
  • Antibody expression
  • ELISA validation
Antibody Purification and Validation
  • Transfection level plasmid preparation
  • Transient transfection expression
  • HTP purification
  • Endotoxin control
Antibody Pairing (optional)
  • ELISA pairing testing
  • Paired antibodies serum sensitivity analysis

Tip 1: Test Methods of PK and ADA Determination

Antigen CaptureAnti-ID CaptureSandwich ELISA
Sketch Diagram
AdvantagesNo need to prepare anti-idiotypic antibodyOnly one anti idiotypic antibody is required Less difficult to developHigh sensitivity
High accuracy
LimitationsLow sensitivity;
Low accuracy
Easy to produce isomorphic cross reactionDifficult development

PK Main Determination Methods

Antigen CaptureAnti-ID Capture
Sketch Diagram
AdvantagesNo need to prepare anti-idiotypic antibodyHigh accuracy
LimitationsLow sensitivity;
Low accuracy
Narrow linear range;
Difficult development

ADA Main Determination Methods

Tip 2: Choosing monoclonal anti-idiotypic antibody or polyclonal anti-idiotypic antibody?

Monoclonal anti-idiotypic antibodies are mainly used to detect the blood concentration of antibody drugs, and can also be used in the evaluation of immunogenicity. Polyclonal anti-idiotypic antibody is mainly used as a positive reference in the immunogenicity study of antibody drugs. It is directly purified from antiserum, which can better simulate the real situation in the blood samples of subjects.

ItemSingleB®Monoclonal Anti-idiotypic AntibodyMonoclonal Anti-idiotypic AntibodyPolyclonal Anti-idiotypic Antibody
AppliactionPK determinationPK determinationImmunogenicity evaluation
Timeline7 weeks20~24 weeks8~12 weeks
AdvantagesHigh specificity;
Short timeline
High specificityBetter imitate situation in blood samples
LimitationsThe immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibodyLonger timeline;
The immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibody
Longer timeline