Idiotype is an antigen-specific marker unique to each antibody molecule, and its epitope is called idiotope. Anti idiotypic antibody (Aid) refers to the antibody that can specifically bind to the unique position of the antibody to be tested in the variable region. Anti idiotypic antibody can specifically detect antibody drugs in the body, and it is the key reagent for pharmacokinetic research (PK) in the process of antibody drug development. In addition, it has many applications in immunogenicity analysis of antibody drugs, vaccine development, ligand neutralization test and antibody blocking test, as well as transplantation immunity, autoimmune diseases, tumor immunity and other fields.
Based on SingleB® antibody development platform, DetaiBio provides the whole process services from antigen preparation, anti idiotypic antibody development to detection kit development and production. The antibody screening process supports the double screening of plasma cells and memory B cells. Compared with the conventional hybridoma monoclonal antibody development, it saves 100 days, and has better antibody diversity, specificity and affinity, ensuring the sensitivity of downstream kit development and lifting the speed limit for antibody drug development.
High Success Rate
Locking specific binding sites in more candidate antibodies
Highly Experienced
Improved kit development platform
Highly Sensitive
The sensitivity meets the needs of preclinical detection
One-stop Service
From antigen preparation to kit development and production
According to the type of antibody drugs to be tested, DetaiBio can provide competitive or non competitive anti idiotypic antibody preparation services. According to the requirements of detection sensitivity, if the detection sensitivity needs to be at the nanogram level, mouse monoclonal anti idiotypic antibody can be selected. If the detection sensitivity needs to be at the peak level, rabbit monoclonal anti idiotypic antibody can be considered.
Workflow | Service | Deliverables |
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Antigen Preparation (optional) | Customers can provide antibody drug sequences or antibody drug samples (purity >85%) |
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Antigen Quality Testing | SDS-PAGE analysis of antibody drug samples | |
Animal Immunization |
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“1+1”Double Screening |
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BGE®HTP Expression & Functional Test |
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Antibody Purification and Validation |
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Antibody Pairing (optional) |
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Antigen Capture | Anti-ID Capture | Sandwich ELISA | |
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Sketch Diagram | ![]() | ![]() | ![]() |
Advantages | No need to prepare anti-idiotypic antibody | Only one anti idiotypic antibody is required Less difficult to develop | High sensitivity High accuracy |
Limitations | Low sensitivity; Low accuracy | Easy to produce isomorphic cross reaction | Difficult development |
PK Main Determination Methods
Antigen Capture | Anti-ID Capture | |
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Sketch Diagram | ![]() | ![]() |
Advantages | No need to prepare anti-idiotypic antibody | High accuracy |
Limitations | Low sensitivity; Low accuracy | Narrow linear range; Difficult development |
ADA Main Determination Methods
Monoclonal anti-idiotypic antibodies are mainly used to detect the blood concentration of antibody drugs, and can also be used in the evaluation of immunogenicity. Polyclonal anti-idiotypic antibody is mainly used as a positive reference in the immunogenicity study of antibody drugs. It is directly purified from antiserum, which can better simulate the real situation in the blood samples of subjects.
Item | SingleB®Monoclonal Anti-idiotypic Antibody | Monoclonal Anti-idiotypic Antibody | Polyclonal Anti-idiotypic Antibody |
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Appliaction | PK determination | PK determination | Immunogenicity evaluation |
Timeline | 7 weeks | 20~24 weeks | 8~12 weeks |
Advantages | High specificity; Short timeline | High specificity | Better imitate situation in blood samples |
Limitations | The immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibody | Longer timeline; The immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibody | Longer timeline |