Anti-idiotype Antibody Production Service
Antibody idiotypes are structural attributes of the V regions of antibody molecules that can be detected by anti-idiotype (anti-ID) antibodies. Since they are capable of binding to antibody drugs within biological fluids, anti-ID antibodies have been used ascritical reagents in pharmacokinetic (PK) and pharmacodynamics (PD) research in antibody drug development. They are also commonly used as key reagents for anti-drug antibody (ADA) studies, vaccine development, transplantation immunity and autoimmune studies, et al.
With SingleB® antibody discovery platform, as described elsewhere in this website, DetaiBio offers antibody discovery of high quality in a fast-and-easy way. Our highly sensitive and specific anti-ID antibodies can help push the boundaries of antibody drug development. This one-stop anti-ID antibody service covers procedures of antigen preparation, anti-ID antibody discovery, antibody pairing, protocol development& transfer and reagents production.
Since rabbit antibodies combine high affinity with high specificity, precise recognition and excellent stability, they hold the potential of overcoming bottlenecking problems such as lower detection limit and heavy matrix effect seen in clinical PK/PD/ADA studies with mouse antibodies, which seriously delays therapeutic antibody development.
DetaiBio provides unique rabbit monoclonal antibody discovery service based on its unparalleled expertise in single B cell screening. With our platform, rabbit antigen-specific memory B cells and plasma cells are directly screened in a single-cell level, with minimal hurt to antibody diversity to guarantee sufficient and high-quality anti-ID antibody generation.
Service Features
High Success Rate
Locking specific binding sites in more candidate antibodies
Highly Experienced
Improved kit development platform
Highly Sensitive
The sensitivity meets the needs of preclinical detection
One-stop Service
From antigen preparation to kit development and production
Types of Anti-idiotype Antibody
Workflow
Monoclonal Anti-idiotype Antibody Production Service
According to the type of antibody drugs to be tested, DetaiBio can provide competitive or non competitive anti idiotypic antibody preparation services. According to the requirements of detection sensitivity, if the detection sensitivity needs to be at the nanogram level, mouse monoclonal anti idiotypic antibody can be selected. If the detection sensitivity needs to be at the peak level, rabbit monoclonal anti idiotypic antibody can be considered.
| Workflow | Service | Deliverables |
|---|
| Antigen Preparation (optional) | Customers can provide antibody drug sequences or antibody drug samples (purity >85%) | - 5-10 ELISA positive clones (ensure delivery of 1 competitive or non competitive antibody)
- Select one strain purified 1 mg antibody
- ELISA paired antibody (optional)
- CoA
|
| Antigen Quality Testing | SDS-PAGE analysis of antibody drug samples |
| Animal Immunization | - 2-4 balb/c mice or 2 rabbits Immunization
- Serum titer test
|
| “1+1”Double Screening | - Memory B cells screening
- Plasma cells screening
|
| BGE®HTP Expression & Functional Test | - Antibody expression
- ELISA validation
|
| Antibody Purification and Validation | - Transfection level plasmid preparation
- Transient transfection expression
- HTP purification
- Endotoxin control
|
| Antibody Pairing (optional) | - ELISA pairing testing
- Paired antibodies serum sensitivity analysis
|
Tip 1: Test Methods of PK and ADA Determination
| Antigen Capture | Anti-ID Capture | Sandwich ELISA |
|---|
| Sketch Diagram |  |  |  |
| Advantages | No need to prepare anti-idiotypic antibody | Only one anti idiotypic antibody is required Less difficult to develop | High sensitivity
High accuracy |
| Limitations | Low sensitivity;
Low accuracy | Easy to produce isomorphic cross reaction | Difficult development |
PK Main Determination Methods
| Antigen Capture | Anti-ID Capture |
|---|
| Sketch Diagram |  |  |
| Advantages | No need to prepare anti-idiotypic antibody | High accuracy |
| Limitations | Low sensitivity;
Low accuracy | Narrow linear range;
Difficult development |
ADA Main Determination Methods
Tip 2: Choosing monoclonal anti-idiotypic antibody or polyclonal anti-idiotypic antibody?
Monoclonal anti-idiotypic antibodies are mainly used to detect the blood concentration of antibody drugs, and can also be used in the evaluation of immunogenicity. Polyclonal anti-idiotypic antibody is mainly used as a positive reference in the immunogenicity study of antibody drugs. It is directly purified from antiserum, which can better simulate the real situation in the blood samples of subjects.
| Item | SingleB®Monoclonal Anti-idiotypic Antibody | Monoclonal Anti-idiotypic Antibody | Polyclonal Anti-idiotypic Antibody |
|---|
| Appliaction | PK determination | PK determination | Immunogenicity evaluation |
| Timeline | 7 weeks | 20~24 weeks | 8~12 weeks |
| Advantages | High specificity;
Short timeline | High specificity | Better imitate situation in blood samples |
| Limitations | The immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibody | Longer timeline;
The immunogenicity evaluation effect is slightly inferior to that of polyclonal anti idiotypic antibody | Longer timeline |
