Anti-idiotype Antibody Production Service

Idiotype is an antigen-specific marker unique to each antibody molecule, and its epitope is called idiotope. Anti idiotypic antibody (Aid) refers to the antibody that can specifically bind to the unique position of the antibody to be tested in the variable region. Anti idiotypic antibody can specifically detect antibody drugs in the body, and it is the key reagent for pharmacokinetic research (PK) in the process of antibody drug development. In addition, it has many applications in immunogenicity analysis of antibody drugs, vaccine development, ligand neutralization test and antibody blocking test, as well as transplantation immunity, autoimmune diseases, tumor immunity and other fields.

Based on SingleB^® antibody development platform, DetaiBio provides the whole process services from antigen preparation, anti idiotypic antibody development to detection kit development and production. The antibody screening process supports the double screening of plasma cells and memory B cells. Compared with the conventional hybridoma monoclonal antibody development, it saves 100 days, and has better antibody diversity, specificity and affinity, ensuring the sensitivity of downstream kit development and lifting the speed limit for antibody drug development.

Service Features

High Success Rate

Locking specific binding sites in more candidate antibodies

Highly Experienced

Improved kit development platform

Highly Sensitive

The sensitivity meets the needs of preclinical detection

One-stop Service

From antigen preparation to kit development and production

Types of Anti-idiotype Antibody

Workflow

Monoclonal Anti-idiotype Antibody Production Service

According to the type of antibody drugs to be tested, DetaiBio can provide competitive or non competitive anti idiotypic antibody preparation services. According to the requirements of detection sensitivity, if the detection sensitivity needs to be at the nanogram level, mouse monoclonal anti idiotypic antibody can be selected. If the detection sensitivity needs to be at the peak level, rabbit monoclonal anti idiotypic antibody can be considered.

Tip 1: Test Methods of PK and ADA Determination

	Antigen Capture	Anti-ID Capture	Sandwich ELISA
Sketch Diagram
Advantages	No need to prepare anti-idiotypic antibody	Only one anti idiotypic antibody is required Less difficult to develop	High sensitivity High accuracy
Limitations	Low sensitivity; Low accuracy	Easy to produce isomorphic cross reaction	Difficult development

PK Main Determination Methods

	Antigen Capture	Anti-ID Capture
Sketch Diagram
Advantages	No need to prepare anti-idiotypic antibody	High accuracy
Limitations	Low sensitivity; Low accuracy	Narrow linear range; Difficult development

ADA Main Determination Methods

Tip 2: Choosing monoclonal anti-idiotypic antibody or polyclonal anti-idiotypic antibody?

Monoclonal anti-idiotypic antibodies are mainly used to detect the blood concentration of antibody drugs, and can also be used in the evaluation of immunogenicity. Polyclonal anti-idiotypic antibody is mainly used as a positive reference in the immunogenicity study of antibody drugs. It is directly purified from antiserum, which can better simulate the real situation in the blood samples of subjects.