Mammalian Transient Expression Service
Mammalian cells have the functions of protein folding and post-translational modification. Compared with prokaryotic expression systems and eukaryotic expression systems such as yeast and insect cells, the recombinant proteins expressed by mammalian cells are closest to natural higher biological protein molecules in terms of molecular structure, physical and chemical properties and biological functions. This characteristic makes mammalian cell expression system popular in the development and production of recombinant protein drugs, especially therapeutic recombinant monoclonal antibodies.
DetaiBio mainly uses CHO (Chinese hamster ovary cells) and HEK293 (human embryonic kidney cells) as expression hosts for suspension culture, and can produce mg level to g level recombinant proteins.
Service Features
Highly Experienced
- More than 10 years of protein expression experience.
- Over 3000 mammalian protein expressed
- Multiple types of protein included
High Success Rate
- CGMP standard cell room
- Multiple proprietary technologies
- Over 98%success rate
Independent R&D
- High expression medium strategy
- High expression vector
- High density cell culture technology
One-stop Service
- One-stop service from codon optimization to protein purification
Workflow
Sequence Analysis & Codon Optimization
1 day
- Customer provides target protein sequence.
- DetaiBio has independently developed MaxCodonTM bioinformatics software to analyze and optimize rare codons, codon preferences and other factors.
Gene Synthesis & Vector Construction
1 week
- Synthetic gene Sequencing validation.
- Cloned into the proprietary high-efficiency expression vector of DetaiBio.
Plasmid Extraction & Transfection Test
1 day
- Determine if the plasmid meets the requirements of transfection
- Determine the appropriate transfection method and host cells
Protein Expression & Purification
1 week
- Select appropriate purification methods and combine with advanced AKTA purification equipment to purify protein
Deliverables:0.5mg protein; CoA; Cloning plasmid 1μg；Gene sequencing report；Service workflow report
Case Study
Case1: Expression and Purification of Tetramer Protein
Lane M: SDS-PAGE Protein Marker
Lane 1: Cell culture supernatant
Lane 2: Effluent
Lane 3: Elution component (reduction)
Lane 4: Eluting component (non reduction)
Case 2: Expression and purification of glycosylated modified protein
Lane M: SDS-PAGE Protein Marker
Lane 1: Cell culture supernatant
Lane 2: Effluent
Lane 3-5: Elution component
Case 3: Expression and purification of high molecular weight protein
Lane M: SDS-PAGE Protein Marker
Lane 1: Cell culture supernatant
Lane 2: Effluent
Lane 3-5: Elution component
How to choose the host cell of mammalian expression system, CHO or HEK293?
| CHO cell | HEK293 cell |
|---|
| Chinese Hamster Ovary Cell | Human Embryonic Kidney 293 Cell |
| Mouse protein, antibody, therapeutic proteins or antibodies are preferred | Proteins or antibodies from human, pig, rabbit and other species are preferred |
| Difficult to transfect with low transfection efficiency | Easy to transfection with high transfection efficiency |
| Transient expression and stable expression | Transient expression |
| Slow cell growth | Rapid cell growth |
| Rarely secretes foreign proteins, conducive to separation and purification | High cell growth density and protein expression |
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