| Product Name | His-Tag Mouse Monoclonal Antibody |
| Type | Monoclonal |
| Product Code | DTH01 |
| Available Sizes | 100 μg, 500 μg, 1 mg |
| Price | Please inquire for pricing |
| Host Species | Mouse |
| Specificity | His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal His-tagged proteins. |
| Clone Type | IgG1 Monoclonal Antibody |
| Purity | >95% Protein A purified |
| Validated Applications | WB, ELISA, IF, IP |
| Immunogen | HHHHHH peptide conjugated to KLH |
| Buffer | PBS, pH 7.4 |
| Storage Conditions | Store at -20°C or below for up to 12 months. Upon arrival, aliquot into appropriate volumes to avoid repeated freeze-thaw cycles. |
| Recommended Dilutions | WB: 1:2500–1:5000 ELISA: 1:2500–1:10000 IF: 1:500 Optimal dilution should be determined experimentally. |
The His-tag, a short peptide consisting of six histidine residues (His-His-His-His-His-His), is commonly fused to the N- or C-terminus of recombinant proteins. It facilitates efficient purification and detection of target proteins without significantly affecting their structure or biological activity, making it one of the most widely used affinity tags in protein engineering.
The His-Tag Mouse Monoclonal Antibody from DETAIBIO specifically recognizes His-tagged proteins with high affinity and excellent dilution performance. It has been extensively validated in multiple applications, including Western Blot (WB), Enzyme-Linked Immunosorbent Assay (ELISA), and Immunofluorescence (IF), and can also be used in immunoprecipitation (IP).
Rapid His Tag Protein Detection Kit